Posts

Showing posts with the label Enzyme-linked immunosorbent assays

Metastability and Eye Movements: A Dynamical‑Systems Interpretation

-
  1. Why metastability is a natural language for gaze behavior The idea that perception, attention, and neural activity evolve through transiently stable states has deep roots in cognitive science and neuroscience. Concepts such as attractors , basins , noise‑induced transitions , and escape times appear in work on perceptual switching, decision‑making, and neural population dynamics (Kelso, 1995; Rabinovich et al., 2008; Deco & Jirsa, 2012). Eye movements, especially the alternation between fixations and saccades ,   fit remarkably well into this metastable picture. The Freidlin–Wentzell theory of rare events (Freidlin & Wentzell, 2012) provides a rigorous mathematical language for these intuitions. 2. Fixations as metastable states  Consider the gaze position X t as the state of a stochastic dynamical system. During a fixation the  gaze remains confined to a small region, microsaccades and noise generate small fluctuations, and the gaze tends to return...
Post a Comment
Read more

Four-parameters logistic function for ELISA-type method

-
Image
Enzyme-linked immunosorbent assays ( ELISA ) are specific and highly sensitive procedures for identifying and quantifying analytes such as proteins in samples. ELISA is routinely used in many areas of biological research, it is based on binding a target molecule (analyte, antigen) to antibodies that recognize the compound. The presence of an antigen-antibody complex is detected using a secondary enzyme-conjugated antibody. Detection is obtained by the addition of a substrate that yields a measurable product. The determination of the analyte concentration relies upon the construction of a calibration curve . The standard curve is prepared by performing a dilution series of known concentrations of the analyte across a range of concentrations near the expected unknown concentration. The calibration curves are then used to calculate the concentration of an unknown sample. For most analyses, a plot of response vs. concentration will create a linear relationship, at least within a certa...
Post a Comment
Read more

Popular posts from this blog

Diagnosing Singular Fits in Linear Mixed Models: Practical Examples, Code, and Alternatives

-
Image
  Introduction This post is the practical follow‑up to When Linear Mixed Models Don’t Converge , where I discussed the theoretical reasons why convergence issues often arise in linear mixed models. Here, we move from theory to practice: simulated examples, diagnostic tools, interpretation of singular fits, and practical modeling alternatives. If you haven’t read the first part yet, you may want to start there for the conceptual foundations. Figure. Conceptual map showing how model complexity and available information interact Legend of Figure 1. Green = identifiable models; yellow = risk of singularity; red = non‑identifiable models. A Concrete Example Using R: When a Random-Slope Model Becomes Singular Let’s simulate a simple dataset with only 8 groups and a very small random‑slope variance. This is a classic scenario where the model converges numerically but is statistically non‑identifiable. ```r set.seed(123) n_groups <- 8 n_per_group <- 20 group <- factor(rep(1:n_gro...
Read more

Understanding Anaerobic Threshold (VT2) and VO2 Max in Endurance Training

-
Image
  Introduction: The Science Behind Ventilatory Thresholds VT1 vs. VT2: Defining Energy Transitions p (Aerobic Threshold) : The transition from lipid metawwwpp (fat-burning) to a mixed metabolism, where both carbohydrates and fats fuel muscle activity. V0T2 (Anaerobic Threshold) : The point where the  shifts to a predominantly carbohydrate-based wmetabolism , leading to rapid lactate waccumpulation and increased reliance on anaerobic energy pathways. www w In p VT1 and VT2 Wwww ewAgep & Sex Younger athletes generally exhibit higher VT2 values , while aging naturally reduces aerobicw capacity. Men tend to have a higher VO2 Max due to greater lung capacity and muscle mass, but women can achieve similar endurance levels through optimized training. Body Composition & Health Status High body fat percentage may reduce VT2 efficiency, as excess weight increases ...
Read more

Unit Root Testing in Practice: A Tutorial on ADF–KPSS, Specification, and Diagnostics

-
Image
  Stationary Signals   A stationary signal is defined by the stability of its statistical properties over time. Specifically, this implies that the signal’s mean, variance, and autocorrelation function remain consistent, regardless of the time in which the signal is evaluated. There are two main types of stationarity: ·        Strict-sense stationarity refers to the property of a signal in which all statistical characteristics remain unchanged over time. This encompasses all moments of the distribution, indicating that the entire probability distribution remains constant throughout the observed period. ·        Wide-sense stationarity requires that the first two moments, specifically the mean and variance, remain constant over time. The autocorrelation function is dependent only on the time difference between two points, not on the exact time points themselves.   Importance in Signal...
Read more